7th semester Instrumental Methods of Analysis

Welcome to the 7th semester Instrumental Methods of Analysis! In this course, we will delve deeper into the principles and techniques used in modern analytical chemistry. We will explore a wide range of instrumental methods that are commonly used for the detection, identification, and quantification of chemical compounds in various sample matrices. Throughout the semester, we will also emphasize the importance of data analysis and interpretation, as well as the application of these methods in real-world scenarios. So, get ready to expand your knowledge and skills in analytical chemistry!


UV Visible Spectroscopy

  • Auxochromes, Chromophores, Beer and Lambert’s law, Electronic transitions, Spectral Shifts, Derivations and Deviations, and Solvent effect on absorption spectra.
  • Instrumentation- Sample cells, Sources of the radiation, Wavelength selectors, Detectors- Silicon Photodiode, Photovoltaic cell, Phototube, and Photomultiplier tube.
  • Applications- Single-component, Spectrophotometric titration, and multi-component analysis.


  • Definitions and Theory, Doublet and triplet electronic states, Concepts of singlet, Factors affecting fluorescence, Internal and External conversation, Quenching, and Instrumentation and application


  •  IR Spectroscopy
  • Introduction and Theory, Factors affecting vibration, Fundamental modes of vibrations in polyatomic molecules, and handling of the sample.
  • Instrumentation Technique– Selectors of wavelength, Sources of radiation, Detectors- Golay cell, Thermistor, Thermocouple, Pyroelectric detector, Bolometer, and their applications.
  • Flame Photometry– Principles and Theory, Interference, Instrumentation and application.
  • Atomic Absorption Spectroscopy– Principles and Theory, Interference, Instrumentation and application.
  • Nepheloturbidometry – Theory and Principles, Instrumentation, and Application.

Unit -3

introduction to Chromatography

• Adsorption and partition column chromatography– Advantages, Disadvantages, Applications, and Methodology.

 Thin Layer Chromatography– Principles, Introduction, Advantages, Disadvantages, Applications, Methodology, and Rf Values.

• Paper Chromatography– Introduction, Advantages, Disadvantages, Applications, Development Techniques, and Methodology.

• Electrophoresis– Introduction, Techniques of paper, gel, capillary electrophoresis, applications, and Factors affecting electrophoretic mobility.


• Gas Chromatography– Theory, Instrumentation, Introduction, Advantages, Disadvantages, Applications, Temperature programming, and Instrumentation.

• HPLC (High-Performance Liquid Chromatography)– Theory, Introduction, Instrumentation, Advantages, Disadvantages, and their Application.


• Ion Exchange Chromatography– Introduction, Classification, Methodology, Applications, mechanism of ion exchange, properties, Ion exchange resins, and factors affecting ion exchange.

• Gel Chromatography– Theory, Introduction, Instrumentation and Application.

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